Ability of Acanthamoeba Cyst to Excyst at Different Temperatures
Abstract
This study was carried out to observe thermotolerance ability of Acanthamoeba spp. A total of 32 Acanthamoeba spp. isolates obtained from water taps, sinks, swimming pools and sea water were used. Trophozoites of Acanthamoeba spp. were inoculated onto non-nutrient agar (NNA) seeded with heat-killed Escherichia coli using aseptic technique and incubated for 14 days at 30°C to obtain the cyst. The cysts were subcultured onto new agar plates for thermotolerance test at 37°C and 42°C. The plates were observed until 96 hours after incubation for excystation of Acanthamoeba before being declared negative. Overall, 81.25% of samples were able to excyst at 37°C while 37.5% were able to excyst at 42°C. Thermotolerant Acanthamoeba is associated with high pathogenicity potential.
Keywords
Thermotolerance; Acanthamoeba cyst; Excystation
Full Text:
PDFRefbacks
- There are currently no refbacks.
Please contact the Chief Editor for any inquiries about the journal. For any technical difficulties please contact our technical support.
eISSN : 2289-4535
ISSN : 1675-8161